abstract：:The effects of class I antiarrhythmic drugs (quinidine, lidocaine, ethmozin) on the maximum upstroke velocity (Vmax) of the action potential (AP) of guinea-pig papillary muscles were investigated in the presence and absence of nifedipine and a potassium-free solution. Nifedipine (10 microM) decreased AP duration from ...

journal_title：Journal of molecular and cellular cardiology

authors： Jurevicius J,Muckus K,Macianskiene R,Chmel-Dunaj GN

更新日期：1991-02-01 00:00:00

abstract：:Monoclonal and polyclonal antibodies to the major sarcoplasmic reticulum proteins of rabbit skeletal and canine cardiac muscle have been used to identify and characterize the corresponding components of human cardiac sarcoplasmic reticulum. The Ca2(+)-transporting ATPase of human cardiac sarcoplasmic reticulum was ide...

journal_title：Journal of molecular and cellular cardiology

authors： Movsesian MA,Leveille C,Krall J,Colyer J,Wang JH,Campbell KP

更新日期：1990-12-01 00:00:00

abstract：:The mechanism of the antiadrenergic action of adenosine in the heart was investigated by examining the effects of phenylisopropyladenosine (PIA), an adenosine A1 receptor agonist, on beta-adrenergic receptor and non-receptor elicited increases in adenylyl cyclase activity of guinea-pig ventricular membranes. These mem...

journal_title：Journal of molecular and cellular cardiology

authors： Romano FD,Dobson JG Jr

更新日期：1990-12-01 00:00:00

abstract：:Acute adriamycin cardiotoxicity was studied in the isolated, perfused rat heart by 31P and 13C NMR spectroscopy at flow rates of 15 and 5 ml/min. Treated hearts received a total dose of 13.5 mg of adriamycin. 31P NMR spectra were collected at the beginning and end of each experiment, and cardiac function was recorded ...

journal_title：Journal of molecular and cellular cardiology

authors： Chatham JC,Cousins JP,Glickson JD

更新日期：1990-10-01 00:00:00

abstract：:Although it is generally accepted that actin and myosin isoforms adapt to their functional requirements, the sequence of expression of these proteins in hearts developing abnormally is unknown. In the chick embryo it is possible to change various aspects of heart development without direct manipulation of the cardiova...

journal_title：Journal of molecular and cellular cardiology

authors： Kirby ML,Shimizu N,Gagnon J,Toyofuku T,Kennedy J,Conrad DC,Zak R

更新日期：1990-09-01 00:00:00

abstract：:Release of atrial natriuretic peptide (ANP) from atrial muscle cells is thought to occur by exocytosis of secretory granules, as in other secretory systems. However, in the atrial myocyte, exocytosis has previously proved difficult to detect ultrastructurally. In order to study the mechanism of ANP release and related...

journal_title：Journal of molecular and cellular cardiology

authors： Newman TM,Severs NJ

更新日期：1990-07-01 00:00:00

abstract：:The possible involvement of protein kinase C in modulating membrane currents was investigated in isolated guinea-pig ventricular cells. In a Na(+)-and K(+)-free external solution, the delayed rectifier K+ current (IK) was increased by the activator of protein kinase C (PKC), 12-O-tetradecanoylphorbol-13-acetate (TPA)....

journal_title：Journal of molecular and cellular cardiology

authors： Tohse N,Kameyama M,Sekiguchi K,Shearman MS,Kanno M

更新日期：1990-06-01 00:00:00

abstract：:Exposure of fine trabeculae isolated from the ferret right ventricle to media depleted of Ca2+, with a rapid perfusion system, causes a depolarization of the membrane and a rise in aiNa. This rise in aiNa is sigmoidally dependent upon the bathing free [Ca2+], is antagonized by reduction of the [Na]o, raised [Mg]o and ...

journal_title：Journal of molecular and cellular cardiology

authors： Bhojani IH,Chapman RA

更新日期：1990-05-01 00:00:00

abstract：:Little is known about the tissue-specific expression of contractile proteins during cardiogenesis in the mammalian heart. Since the myosin heavy chain (HC) isoform expressed in the adult correlates with myocardial functional capacity, we undertook an analysis of myosin HC expression in atrial and ventricular myocardia...

journal_title：Journal of molecular and cellular cardiology

authors： Sweeney LJ,Kelley SW

更新日期：1990-03-01 00:00:00

abstract：:The aim of the present study was to investigate whether or not alterations of Gs alpha can be detected with cholera toxin-induced ADP-ribosylation in myocardial membranes from patients with heart failure. Therefore, Gs alpha was radiolabeled by cholera toxin-catalzyed (32P)ADP-ribosylation with (32P)NAD as substrate. ...

journal_title：Journal of molecular and cellular cardiology

authors： Schnabel P,Böhm M,Gierschik P,Jakobs KH,Erdmann E

更新日期：1990-01-01 00:00:00

abstract：:We studied post-extrasystolic potentiation (PESP) and frequency potentiation (FP) of human working myocardium isolated from the ventricles of 10 patients with end-stage heart failure (CHF) and 17 non-failing controls (CTL). The contractility index was peak isometric tension developed in vitro by trabeculae carneae (CT...

journal_title：Journal of molecular and cellular cardiology

authors： Phillips PJ,Gwathmey JK,Feldman MD,Schoen FJ,Grossman W,Morgan JP

更新日期：1990-01-01 00:00:00

abstract：:(1) The effects of norepinephrine on protein phosphorylation in isolated rat cardiac ventricular myocytes were determined by autoradiography on 32P-labelled proteins separated by electrophoresis; (2) In cells from young adult rats (6 months old) there was a marked increase due to norepinephrine (10(-8) to 10(-4) M) in...

journal_title：Journal of molecular and cellular cardiology

authors： Sakai M,Danziger RS,Staddon JM,Lakatta EG,Hansford RG

更新日期：1989-12-01 00:00:00

abstract：:Ischemia-induced myocardial potassium loss and post-ischemic potassium reuptake was quantitated in 8 open chest pigs during control conditions and during hemodynamic alterations which have been shown to increase steady state sarcolemmal potassium fluxes. Myocardial K+ balance was continuously computed before, during a...

journal_title：Journal of molecular and cellular cardiology

authors： Aksnes G,Ellingsen O,Rutlen DL,Ilebekk A

更新日期：1989-12-01 00:00:00

abstract：:Echocardiographic dimensions and endocrinological examination were performed in 71 young patients with borderline hypertension (BHT) and 31 age-matched normotensive subjects (NT). Patients with BHT had significantly higher relative wall thickness (RWT) and left ventricular mass index than NT, although all BHT patients...

journal_title：Journal of molecular and cellular cardiology

authors： Fujita T,Noda H,Ito Y,Isaka M,Sato Y,Ogata E

更新日期：1989-12-01 00:00:00

abstract：:To examine cardiac myosin gene structure and expression in a non-human primate model for human heart development and disease, we have constructed a cDNA library from baboon atrium and used baboon beta-myosin heavy chain (beta-MHC)* cDNA probes to isolate atrial MHC clones. The nucleotide sequence of one such clone, la...

journal_title：Journal of molecular and cellular cardiology

authors： Hixson JE,Henkel RD,Britten ML,Vernier DT,deLemos RA,VandeBerg JL,Walsh RA

更新日期：1989-10-01 00:00:00

abstract：:Reoxygenation of isolated rat cardiac myocytes following a period of hypoxia and substrate deprivation resulted in a 1.5-2-fold increase in the total Ca2+ content which could be inhibited by 1 microM antimycin A or ruthenium red (50% inhibition at 2.5 microM). This increase in Ca2+ content was not accompanied by any r...

journal_title：Journal of molecular and cellular cardiology

authors： Stone D,Darley-Usmar V,Smith DR,O'Leary V

更新日期：1989-10-01 00:00:00

abstract：:The regulation of and the intracellular events following alpha 1-adrenergic receptor stimulation of myocardium are not completely understood. The alpha 1-adrenergic stimulation of phosphoinositide breakdown was examined in a culture of neonatal rat ventricular myocytes and the influence of a protein kinase C activator...

journal_title：Journal of molecular and cellular cardiology

authors： Meij JT,Lamers JM

更新日期：1989-07-01 00:00:00

abstract：:The metabolism of pantothenic acid (Pa) by cardiac muscle was studied in normal and diabetic rats. Tissue levels of Coenzyme A (CoA) are elevated in the heart during early (6 to 12 h) diabetes, remains at a high level for several days, and then returns to normal or below normal levels. The increase in total tissue CoA...

journal_title：Journal of molecular and cellular cardiology

authors： Beinlich CJ,Robishaw JD,Neely JR

更新日期：1989-07-01 00:00:00

abstract：:The relationship between reperfusion-induced arrhythmias and the size of the occluded zone was examined. The isolated perfuse rat heart was used because its negligible collateral flow maximizes susceptibility to arrhythmias and reduces variability. Ischemia lasting 10 min was followed by 10 min of reperfusion. A const...

journal_title：Journal of molecular and cellular cardiology

authors： Curtis MJ,Hearse DJ

更新日期：1989-06-01 00:00:00

abstract：:Although the myocardium is capable of utilizing both glucose and fatty acid substrates, glucose metabolism is inhibited in the presence of fatty acid during normal perfusion conditions. Fatty acid regulation of glucose utilization in intact beating rat hearts was studied with 13C-enriched substrates and 13C and 31P NM...

journal_title：Journal of molecular and cellular cardiology

authors： Weiss RG,Chacko VP,Gerstenblith G

更新日期：1989-05-01 00:00:00

abstract：:Interactions between platelets with injured vascular endothelium contribute to thrombotic occlusion. A murine monoclonal antibody [7E3 F(ab')2] to the platelet GPIIb/IIIa receptor complex was used to inhibit platelet aggregation in an experimental model of coronary artery thrombosis. Prevention of thrombotic occlusion...

journal_title：Journal of molecular and cellular cardiology

authors： Mickelson JK,Simpson PJ,Lucchesi BR

更新日期：1989-04-01 00:00:00

abstract：:The objective of this study was to characterize the effect of prostacyclin (PGI2) on ventricular function following total global ischemia in isolated working rat hearts and to investigate the mechanism of its action. Ischemia was initiated for 10, 15, 20 or 25 min with or without treatment with PGI2. Increasing durati...

journal_title：Journal of molecular and cellular cardiology

authors： Karmazyn M,Neely JR

更新日期：1989-03-01 00:00:00

abstract：:Energy metabolism of quiescent cardiac muscle was studied in the isolated rabbit heart preparation perfused at constant pressure by the Langendorff technique. Oxygen consumption (MVo2), coronary flow rate (CFR) and the steady state concentrations of high energy phosphate compounds were determined in hearts rendered as...

journal_title：Journal of molecular and cellular cardiology

authors： Kotsanas G,Gibbs CL,Wendt IR

更新日期：1989-02-01 00:00:00

abstract：:Endothelial cells responses to a variety of agonists include release of endothelium dependent vasodilators, such as endothelium dependent relaxing factor (EDRF) and prostacyclin (PGI2). These substances act on vascular smooth muscle to cause relaxation and also have potent anti-aggregatory effects on platelets. A stud...

journal_title：Journal of molecular and cellular cardiology

authors： Ryan US

更新日期：1989-02-01 00:00:00

abstract：:Left ventricular pressure overload will result in the hypertrophic growth of the myocardium and in the rat may include a remodeling of both the structural and contractile proteins. As a result, an adaptive rise in active stiffness, or the force generating capacity of the myocardium, may occur. The relative importance ...

journal_title：Journal of molecular and cellular cardiology

authors： Jalil JE,Doering CW,Janicki JS,Pick R,Clark WA,Abrahams C,Weber KT

更新日期：1988-12-01 00:00:00

abstract：:The direct effect of oxygen metabolites was studied on isolated perfused rat hearts. Superoxide anion (O2-.) and hydrogen peroxide (H2O2) were generated by adding purine (2.3 mM) and purified xanthine oxidase (0.06 U/ml) to Krebs-Henseleit buffer (pH 7.4). Xanthine oxidase was added to the purine-containing perfusate ...

journal_title：Journal of molecular and cellular cardiology

authors： Miki S,Ashraf M,Salka S,Sperelakis N

更新日期：1988-11-01 00:00:00

abstract：:The ability of the beta-receptor antagonist propranolol to influence the response of isolated cardiac and vascular smooth muscle to several classes of calcium channel blockers was examined. For comparison, the interactions between propranolol and other classes of negative inotropic and vasorelaxant agents was also eva...

journal_title：Journal of molecular and cellular cardiology

authors： Weishaar RE,Quade MM,Pugsley TA,Shih YH,Taylor DG Jr

更新日期：1988-10-01 00:00:00

abstract：:We studied the oxygen diffusion distance in the rabbit myocardium in untreated animals and in animals treated with thyroxine (T4) for 3 days and 16 days. The subepicardial and subendocardial regions were studied separately. Sixteen days of T4 treatment results in significant cardiac hypertrophy. After 16 days, the per...

journal_title：Journal of molecular and cellular cardiology

authors： Seiden D,Navidad P,Weiss HR

更新日期：1988-10-01 00:00:00

abstract：:The purpose of this study was to determine whether thyroid hormone could directly affect the phenotypic expression of two isozymic systems [lactate dehydrogenase (LDH) and myosin] and the energy transducing potential of cultured neonatal heart cells. In addition we determined if these biochemical systems developed in ...

journal_title：Journal of molecular and cellular cardiology

authors： Williams HM,Ianuzzo CD

更新日期：1988-08-01 00:00:00

abstract：:Cardiomyopathy of the Syrian hamster is characterized by myocardial calcium overload and focal myocardial necrosis. The cause of the myocardial calcium overload is not yet fully understood. In this study, the ultrastructural localization of calcium was determined in normal hamster hearts and in non-necrotic and necrot...

journal_title：Journal of molecular and cellular cardiology

authors： Olbrich HG,Borgers M,Thoné F,Frotscher M,Mutschler E,Schneider M,Kober G,Kaltenbach M

更新日期：1988-08-01 00:00:00

abstract：:In addition to the well-known reduction in the maximal rate of rise the antiarrhythmic agent, lidocaine, also produces a shortening in the duration of the normal ventricular action potential. The voltage-dependent currents carried by Na, Ca and K ions were examined in the absence and presence of lidocaine, in order to...

journal_title：Journal of molecular and cellular cardiology

authors： Josephson IR

更新日期：1988-07-01 00:00:00

abstract：:Myocytes were isolated by Langendorff perfusion of rat or rabbit hearts with low calcium solution followed by collagenase and hyaluronidase, or by incubation of chunks of rat ventricular tissue in similar media. Cells were then placed in a bath on a microscope stage, superfused and electrically stimulated. Contraction...

journal_title：Journal of molecular and cellular cardiology

authors： Harding SE,Vescovo G,Kirby M,Jones SM,Gurden J,Poole-Wilson PA

更新日期：1988-07-01 00:00:00

abstract：:Readmission of Ca2+ after a short period of Ca2+-free perfusion results in a rapid and massive release of cytoplasmic proteins from the heart (calcium paradox). Maximal release rates of proteins are already reached within 2 min after Ca2+ repletion. The precise mechanism underlying the loss of cellular membrane integr...

journal_title：Journal of molecular and cellular cardiology

authors： van der Vusse GJ,van Bilsen M,Willemsen P,Reneman RS

更新日期：1988-07-01 00:00:00

abstract：:To determine whether catecholamines produce alterations in myocardial myosin-actin cycling kinetics, we investigated the effects of isoproterenol upon mechanical characteristics of constantly activated heart muscle thought to reflect crossbridge behavior. In isolated rabbit right ventricular papillary muscles in bariu...

journal_title：Journal of molecular and cellular cardiology

authors： Berman MR,Peterson JN,Yue DT,Hunter WC

更新日期：1988-05-01 00:00:00

abstract：:Nitrovasodilators relax vascular smooth muscle by stimulating guanylate cyclase. Ignarro et al. (1981) proposed a mechanistic scheme according to which organic nitrates release nitrite in the presence of thiols. The corresponding nitrous acid would decay leading to nitric oxide, which then would react with another thi...

journal_title：Journal of molecular and cellular cardiology

authors： Romanin C,Kukovetz WR

更新日期：1988-05-01 00:00:00

abstract：:The ultrastructure of myocardial tissue was studied during ischemia and reperfusion and during reperfusion with a calcium-containing solution after a short period of calcium-free perfusion (calcium paradox). After ischemia an aggregation of the sarcolemmal intramembranous particles was observed. Subsequent reperfusion...

journal_title：Journal of molecular and cellular cardiology

authors： Post JA,Ruigrok TJ,Verkleij AJ

更新日期：1988-03-01 00:00:00

abstract：:Global ischemia in guinea-pig hearts for 60 to 90 min depressed microsomal and mitochondrial Ca2+ uptake activities. Reperfusion of the 60 min ischemic hearts resulted in incomplete recovery of contractile function and calcium uptake activities of both mitochondrial and microsomal fractions. On the other hand, reperfu...

journal_title：Journal of molecular and cellular cardiology

authors： Dhalla NS,Panagia V,Singal PK,Makino N,Dixon IM,Eyolfson DA

更新日期：1988-03-01 00:00:00

abstract：:The effect of linoleic and arachidonic acid derivatives on ATP-dependent calcium transport was studied in the isolated vesicles from cardiac sarcoplasmic reticulum of guinea-pigs. Oxidation products of linoleic and arachidonic acids, obtained either by autoxidation or incubation with soybean lipoxygenase, effectively ...

journal_title：Journal of molecular and cellular cardiology

authors： Kim RS,LaBella FS

更新日期：1988-02-01 00:00:00

abstract：:Carnitine, certain of its derivatives, and the amino acid metabolite, taurine, when administered independently in prior studies have been shown to improve cardiac mechanic and/or metabolism. The purpose of these studies is to test a new compound, propionylcarnitine taurine (PCT), which potentially combines these actio...

journal_title：Journal of molecular and cellular cardiology

authors： Molaparast-Saless F,Nellis SH,Liedkte AJ

更新日期：1988-01-01 00:00:00

abstract：:The ability of cells in anoxic-perfused hearts to withstand two types of physical stresses (stretching and swelling), in the absence of reoxygenation was determined. Isolated rat hearts were perfused for 20, 40, or 60 mins with anoxic buffer after which hearts were either reoxygenated for 20 mins or exposed for 15 s t...

journal_title：Journal of molecular and cellular cardiology

authors： Vander Heide RS,Ganote CE

更新日期：1987-11-01 00:00:00